Investigating Enzyme Activity :: Papers

Investigating Enzyme Activity PLANNING AIM === The aim of this experiment is to see how changing the concentration of Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. The reaction I am looking at is the breakdown of Hydrogen Peroxide into water and oxygen by the enzyme Catalase. The equation for this reaction is: 2H2O2 Ã   2H2O + O2 FACTORS ------- In this experiment I could change the following factors: Concentration of the Hydrogen Peroxide or Catalase, the time, the temperature and the Ph of the solutions. SCIENTIFIC KNOWLEDGE (See appendix 1) PREDICTION ---------- My prediction is that the higher the concentration of Hydrogen Peroxide, the greater the amount of oxygen given off will be within a period of time. The lower the concentration of Hydrogen Peroxide the amount of oxygen given off will be less than if it was at a higher concentration. I think this because there are more particles in a substance to collide and react at a higher concentration. VARIABLES I am not going to change anything other than the concentration of the Hydrogen Peroxide. The concentration of the Catalase enzyme, the temperature of the laboratory and the Ph of the solutions will remain constant. EQUIPMENT Hydrogen Peroxide (concentration - 0.5 1.0%, 1.5%, 2.0%, 2.5% and 3.0%) Safety Catalase Water trough, Burette, Stop clock, Delivery tube, Clamp stand, Conical flask, Syringe, Pipette Glasses/goggles, PROCEDURE 1. I will take the equipment drawn and set it up as shown. *(appendix 2) 2. I will fill the burette and water trough with water. 3. Using a measuring cylinder, I will put 10ml of Hydrogen Peroxide in the conical flask connected to the delivery tube. 4. Trying to prevent water from escaping, I shall turn the burette upside down and clamp it to the stand with the bottom of the burette in the water trough and feed the delivery tube through the burette.